Structural Characterization of Lignin in Wild-type versus COMT Down-regulated Switchgrass

Structural Characterization of Lignin in Wild-type versus COMT Down-regulated Switchgrass

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This study examined the chemical structural characteristics of cellulolytic enzyme lignin isolated from switchgrass focusing on comparisons between wild-type control and caffeic acid 3-O-methyltransferase (COMT) down-regulated transgenic line.Nuclear magnetic resonance (NMR) techniques including 13C, 31P, and two-dimensional 13C-1H heteronuclear single quantum coherence (HSQC) canine spectra kc 3 intranasal single dose as well as gel permeation chromatography (GPC) were employed.Compared to the wild-type, the COMT down-regulated transgenic switchgrass lignin demonstrated a decrease in syringyl (S): guaiacyl (G) ratio and p-coumarate:ferulate ratio, an increase in relative abundance of phenylcoumaran unit, and a comparable dea eyewear content of total free phenolic OH groups along with formation of benzodioxane unit.In addition, COMT down-regulation had no significant effects on the lignin molecular weights during its biosynthesis process.